Azido-PEG4-NHS Ester疊(die)(die)氮(dan)基-PEG4-NHS 酯是一種標(biao)記試劑，可用于(yu)通(tong)過穩定的(de)(de)酰胺(an)鍵修飾具有(you)疊(die)(die)氮(dan)基團(tuan)的(de)(de)伯胺(an)基團(tuan)（例(li)如賴氨酸殘基的(de)(de)側鏈(lian)或氨基硅烷包被的(de)(de)表面）。疊(die)(die)氮(dan)化(hua)物 （N3） 基團(tuan)通(tong)過 CuAAC 與末端炔(gui)烴反(fan)應(ying)反(fan)應(ying)，通(tong)過無(wu)銅(tong)點擊反(fan)應(ying)與應(ying)變環辛(xin)炔(gui)（例(li)如 DBCO 或 BCN 化(hua)合(he)物）反(fan)應(ying)，或通(tong)過稱為 Staudinger 化(hua)學的(de)(de)機制與磷化(hua)氫標(biao)記分子反(fan)應(ying)，從而實(shi)現(xian)生(sheng)物樣品中(zhong)衍生(sheng)化(hua)分子的(de)(de)高效(xiao)和特(te)異性偶聯。

Azido-PEG4-NHS Ester is a labeling reagent that can be used to modify primary amine groups (e.g., a side chain of lysine residues or aminosilane-coated surfaces) with an azido group via a stable amide bond. The azide (N3) group reacts with terminal alkynes via CuAAC, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.