生(sheng)物(wu)素-PEG3-疊(die)氮化(hua)物(wu)是一種不可裂解的(de)(de)(de)疊(die)氮化(hua)物(wu)活化(hua)生(sheng)物(wu)素化(hua)試劑，通(tong)過(guo)銅(tong)催(cui)化(hua)的(de)(de)(de)點擊(ji)反應(ying)與(yu)末(mo)端炔烴反應(ying)反應(ying)，通(tong)過(guo)無銅(tong)點擊(ji)反應(ying)與(yu)應(ying)變的(de)(de)(de)環(huan)辛炔（例如DBCO或(huo)BCN化(hua)合物(wu)）反應(ying)，或(huo)通(tong)過(guo)稱為Staudinger化(hua)學(xue)的(de)(de)(de)機制與(yu)磷化(hua)氫標記的(de)(de)(de)分(fen)子反應(ying)，從(cong)而實現生(sheng)物(wu)樣品中(zhong)衍(yan)生(sheng)化(hua)分(fen)子的(de)(de)(de)高效和特異性偶聯。這些反應(ying)是生(sheng)物(wu)正交的(de)(de)(de)，或(huo)者換(huan)句話說，與(yu)生(sheng)物(wu)系統相(xiang)容(rong)，因為它的(de)(de)(de)成分(fen)不與(yu)生(sheng)物(wu)環(huan)境發(fa)生(sheng)反應(ying)。

Biotin-PEG3-Azide is non-cleavable, azide-activated biotinylation reagent that reacts with terminal alkynes via a copper-catalyzed click reaction, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. These reactions are bioorthogonal or in other words compatible with biological systems in that it′s components do not react with the biological environment.