BTTAA 是最(zui)新一(yi)代的(de)(de)(de)(de) CuAAC 水溶(rong)性加(jia)速配體(ti),與上一(yi)代配體(ti)(例如 THPTA 或(huo) TBTA)相比(bi),它(ta)提供了(le)更大的(de)(de)(de)(de)速率(lv)增強。更重要的(de)(de)(de)(de)是,它(ta)最(zui)大限度(du)地(di)減(jian)少了(le)對所探測細胞(bao)或(huo)生物體(ti)生理狀態的(de)(de)(de)(de)擾動,并通(tong)過進一(yi)步降低催化(hua)劑配方中的(de)(de)(de)(de)銅負載量(liang),實現了(le)有(you)效的(de)(de)(de)(de)生物偶聯,抑制了(le)細胞(bao)毒(du)性。
BTTAA is a newest generation, water-soluble accelerating ligand for CuAAC that provides much greater rate enhancement compared to previous generation ligands (e.g. THPTA or TBTA). More importantly, it minimizes perturbations to the physiological state of the cells or organisms probed and allows for effective bioconjugation with suppressed cell cytotoxicity by further lowering copper loading in the catalyst formulation.