生(sheng)物素(su)(su)-親和素(su)(su)相互(hu)作(zuo)用(yong)通常用(yong)于檢測和/或純化蛋(dan)白(bai)質(zhi)，因為這(zhe)兩種分子(zi)彼此具(ju)有高度特異(yi)性。生(sheng)物素(su)(su)化是(shi)將生(sheng)物素(su)(su)附(fu)著(zhu)在蛋(dan)白(bai)質(zhi)和其(qi)他(ta)大分子(zi)上(shang)的過程。

在選(xuan)擇生物素化(hua)試劑時，用戶需要(yao)考(kao)慮以下幾個方(fang)面。

The biotin–avidin interaction is commonly exploited to detect and/or purify proteins because of the high specificity that these two molecules have for each other. Biotinylation is the process of attaching biotin to proteins and other macromolecules.

When selecting the biotinylating reagents, the user need consider the following aspects.

BroadPharm has all kinds of biotinlyation reagents for meet the need of your advanced research. The variability of these reagents substantially expands the range of applications for avidin–biotin chemistry

• *Varieties of biotin functional group reagents differ in length, solubility, cell permeability and cleavability.

• *Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.

• *Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF.

• *Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable.

BroadPharm擁有各(ge)種生物(wu)(wu)素(su)化試劑，以滿足您高級(ji)研究的需(xu)求。這些試劑的可變性大(da)大(da)擴展了(le)親和素(su)-生物(wu)(wu)素(su)化學的應用(yong)范圍

*各(ge)種生物素官能團試劑的長度(du)、溶解度(du)、細(xi)胞通透性(xing)和裂解性(xing)各(ge)不相同。

*含有二(er)硫鍵的品種(zhong)可(ke)以使用還原(yuan)劑進行(xing)裂(lie)解，使生物素基團能夠與(yu)標記(ji)的蛋白質(zhi)斷開。

*非磺化NHS-生物(wu)素(su)具有(you)細胞滲透性，但必須溶(rong)解在有(you)機溶(rong)劑(ji)中，如(ru)DMSO或(huo)DMF。

*Sulfo-NHS生物(wu)素（以及那些帶有聚乙(yi)二醇化間(jian)隔物(wu)的生物(wu)素）是直(zhi)接水溶(rong)性(xing)的，但不能滲透膜。