您是否希望(wang)懸浮(fu)細(xi)(xi)胞的(de)免疫(yi)熒光(guang)像(xiang)貼壁細(xi)(xi)胞一(yi)樣容易?現在(zai)是這樣!Shi-fix™(由Shikhar生物技術公司制造)蓋滑允許懸浮(fu)細(xi)(xi)胞分層(ceng)或(huo)直(zhi)接作為(wei)單層(ceng)生長。簡單,無(wu)憂的(de)實(shi)驗(yan)方案,無(wu)需離心柱即可將懸浮(fu)細(xi)(xi)胞固定到(dao)載玻(bo)片(pian)上。只需將細(xi)(xi)胞添加到(dao)蓋玻(bo)片(pian)中,等待30分鐘,用PBS洗滌未(wei)結合(he)的(de)細(xi)(xi)胞并(bing)繼(ji)續免疫(yi)染色(或(huo)繼(ji)續培養以獲得所需的(de)細(xi)(xi)胞密(mi)度)。
這些蓋(gai)玻片也可(ke)用(yong)于染色懸(xuan)浮細(xi)(xi)胞(bao)以進(jin)(jin)行顯(xian)微(wei)鏡檢(jian)查(cha),或用(yong)于固定懸(xuan)浮細(xi)(xi)胞(bao)以進(jin)(jin)行共聚(ju)焦(jiao)顯(xian)微(wei)鏡檢(jian)查(cha)。更重(zhong)要(yao)的(de)是,如果需要(yao),您還可(ke)以在細(xi)(xi)胞(bao)附著在蓋(gai)玻片上時(shi)刺激(ji)它們。您可(ke)以像貼壁細(xi)(xi)胞(bao)一樣處理非貼壁細(xi)(xi)胞(bao)!
Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!
SHI-FIX cover-slips玻片做的懸浮細胞免疫熒光實驗圖:
SHI-FIX cover-slips玻片的操作步驟:簡單!方便!!
SHI-FIX cover-slips玻片的產品訂購信息: